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Endothelial cell proliferation – Fully booked

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Hybrid course (seminar & workshop) including hands-on experience in handling human endothelial cells and their acute responses to growth factors. Includes experiments with live cell imaging (IncuCyte) and EdU incorporation as well as data analysis.

Vascular inflammation – Fully booked

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Hybrid course (seminar & workshop) including hands-on experience in adherence assays (endothelial cells & monocytes) and imaging.

FACS Analysis – Fully Booked

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Introduction to flow cytometry and the Fortessa X-20 flow cytometer. Hands on experience in sample preparation, staining and analysis of different types of cells death.

Vascular inflammation

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Hybrid course (seminar & workshop) including hands-on experience in adherence assays (endothelial cells & monocytes) and imaging.

FACS Analysis – Fully booked

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Introduction to flow cytometry and the Fortessa X-20 flow cytometer. Hands on experience in sample preparation, staining and analysis of different types of cells death.

Endothelial cell proliferation

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Hybrid course (seminar & workshop) including hands-on experience in handling human endothelial cells and their acute responses to growth factors. Includes experiments with live cell imaging (IncuCyte) and EdU incorporation as well as data analysis.

Targeted metabolomics: analysis of amino acids and secondary amines

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Theoretical and practical introduction to Metabolomics, including amine group derivatisation, sample extraction, MS run, amine identification and basic stats

GPCR cAMP Signalling for beginners

MPI Bad Nauheim Ludwigstraße 43, Bad Nauheim, Deutschland

Course content: Theoretical introduction of diverse GPCR signalling and assay formats. Practical session on performing cAMP assay in HEK cells

Foundation course in sample preparation and basic confocal microscopy

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Introduction to microscope adjustment and choice of objectives. Sample fixation, demasking, antibody labelling. Basic use of a confocal microscope using pre-prepared and student prepared samples - troubleshooting

GPCR cAMP Signalling for beginners

MPI Bad Nauheim Ludwigstraße 43, Bad Nauheim, Deutschland

Course content: Theoretical introduction of diverse GPCR signalling and assay formats. Practical session on performing cAMP assay in HEK cells

Foundation course in sample preparation and basic confocal microscopy

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Introduction to microscope adjustment and choice of objectives. Sample fixation, demasking, antibody labelling. Basic use of a confocal microscope using pre-prepared and student prepared samples - troubleshooting

The general principles of mass spectrometry

University Hospital Frankfurt, Building 25B Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Theoretical introduction into the basics of mass spectrometry and different proteomic and metabolomic approaches.

Visit to the hospital catheter laboratory – Fully booked

University Hospital Frankfurt, Cardiology Theodor-Stern-Kai 7, Frankfurt am Main, Deutschland

Course content: Introduction to therapeutic strategies for patients with acute coronary syndromes. Demonstration of examinations in the catheter laboratory.

Expansion microscopy of immunostained tissue

Biologicum Max-von-Laue-Str. 13, Frankfurt am Main, Deutschland

Course content: demonstrates a model of organ culture (4 days) to monitor pericyte escape from the retinal vasculature. Please note: On 23 January the course will only take 1/2 day.

Leukocyte adhesion and transmigration

MPI Bad Nauheim Ludwigstraße 43, Bad Nauheim, Deutschland

Course content: Theoretical and practical introduction to different assays analyzing leukocyte adhesion to human endothelial cells as well as their transmigration in the transwell system.

Leukocyte adhesion and transmigration

MPI Bad Nauheim Ludwigstraße 43, Bad Nauheim, Deutschland

Course content: Theoretical and practical introduction to different assays analyzing leukocyte adhesion to human endothelial cells as well as their transmigration in the transwell system.

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